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Western blot analysis of extracts of MCF7 cells, using Phospho-Tp53-S6 antibody (AP0951) at 1:1000 dilution.MCF7 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-p53-S6 Rabbit pAb
Catalog No：AB-AP0951
Manufacturer：ABclonal
Synonyms：BCC7;LFS1;P53;TRP53;p53;TP53;BMFS5
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p53-S6 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S6 of human p53. |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 43kDa |
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