Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-p53-S15 antibody (AP0083). Green锛欸FP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence analysis of U2OS cells using Phospho-p53-S15 antibody (AP0083). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-p53-S15 pAb (AP0083). Western blot was performed from the immunoprecipitate using Phospho-p53-S15 pAb (AP0083) at a dilition of 1:1000. 293T cells were treated by UV at room temperature for 30 minutes after serum-starvation overnight, and then treated by 10% FBS at 37℃ for 30 minutes.

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S15 antibody ( AP0083) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.