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Immunofluorescence analysis of U2OS cells using Phospho-p38 MAPK-Y322 antibody (AP0055). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 293 and NIH/3T3 cells, using Phospho-p38 MAPK-Y322 antibody (AP0055) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25ug/mL) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Phospho-p38 MAPK-Y322 Rabbit pAb
Catalog No：AB-AP0055
Manufacturer：ABclonal
Synonyms：MAPK14;CSBP;CSBP1;CSBP2;CSPB1;EXIP;Mxi2;PRKM14;PRKM15;RK;SAPK2A;p38;p38ALPHA;p38 MAPK
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p38 MAPK-Y322 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Immunogen: | A synthetic phosphorylated peptide around Y322 of human p38 MAPK (NP_620581.1). |
| Gene ID: | 1432 |
| Swiss prot: | Q16539 |
| Molecular weight: | 29kDa/34kDa/35kDa/41kDa |
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