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Western blot analysis of extracts of Jurkat cells, using Phospho-NCF4-T154 antibody (AP1193) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-NCF4-T154 Rabbit pAb
Catalog No：AB-AP1193
Manufacturer：ABclonal
Synonyms：NCF4;CGD3;NCF;P40PHOX;SH3PXD4
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-NCF4-T154 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Immunogen: | A synthetic phosphorylated peptide around T154 of human NCF4. |
| Gene ID: | 4689 |
| Swiss prot: | Q15080 |
| Molecular weight: | 39kDa |
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