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Western blot analysis of extracts of 293T cells, using Phospho-Na+/K+-ATPase-S16 antibody (AP1065) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Na+/K+-ATPase-S16 Rabbit pAb
Catalog No：AB-AP1065
Manufacturer：ABclonal
Synonyms：ATP1A1;ATPase Na+/K+ transporting subunit alpha 1;ATP1A;CMT2DD;HOMGSMR2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Na+/K+-ATPase-S16 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S16 of human Na+/K+-ATPase. |
| Gene ID: | 476 |
| Swiss prot: | P05023 |
| Molecular weight: | 112kDa |
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