Western blot analysis of extracts of C2C12 cells, using Phospho-mTOR-S2481 antibody (AP0490) at 1:1000 dilution.C2C12 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 5min.
Phospho-mTOR-S2481 Rabbit pAb
Catalog No:AB-AP0490
Manufacturer:ABclonal
Synonyms:FRAP;FRAP1;FRAP2;RAFT1;RAPT1;SKS;mTOR;MTOR
Applications:WB
Reactivity:Human,Mouse,
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-mTOR-S2481 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
Immunogen: | A synthetic phosphorylated peptide around S2481 of human mTOR (NP_004949.1). |
Gene ID: | 2475 |
Swiss prot: | P42345 |
Molecular weight: | 288kDa |
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