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Western blot analysis of extracts of various cell lines, using Phospho-mTOR-S2481 (AP0978) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-mTOR-S2481 Rabbit mAb
Catalog No：AB-AP0978
Manufacturer：ABclonal
Synonyms：FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-mTOR-S2481 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S2481 of human mTOR |
| Gene ID: | 2475 |
| Swiss prot: | P42345 |
| Molecular weight: | 289kDa |
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