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Western blot analysis of extracts of 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (AP0115) at 1:1000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-mTOR-S2448 Rabbit mAb
Catalog No：AB-AP0115
Manufacturer：ABclonal
Synonyms：FRAP;FRAP1;FRAP2;RAFT1;RAPT1;SKS;mTOR;MTOR
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-mTOR-S2448 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008] |
| Immunogen: | A synthesized peptide derived from human Phospho-mTOR (S2448). |
| Gene ID: | 2475 |
| Swiss prot: | P42345 |
| Molecular weight: | 289kDa |
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