Western blot analysis of extracts of C6 cells, using Phospho-MEK1-T292 Rabbit mAb (AP1021) at 1:1000 dilution.C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-T292 pAb (AP1021) at 1:1000 dilution or MAP2K1 antibody (A12687).HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-MEK1-T292 Rabbit mAb
Catalog No:AB-AP1021
Manufacturer:ABclonal
Synonyms:CFC3, MAPKK1, MEK1, MKK1, PRKMK1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-MEK1-T292 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T292 of human Phospho-MEK1-T292 |
Gene ID: | 5604 |
Swiss prot: | Q02750 |
Molecular weight: | 45kDa |
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