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Western blot analysis of extracts of various cell lines, using Phospho-MEK1-S298 Rabbit mAb (AP1020) at 1:1000 dilution.Both HeLa cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
Phospho-MEK1-S298 Rabbit mAb
Catalog No：AB-AP1020
Manufacturer：ABclonal
Synonyms：CFC3, MAPKK1, MEK1, MKK1, PRKMK1
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-MEK1-S298 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S298 of human Phospho-MEK1-S298 |
| Gene ID: | 5604 |
| Swiss prot: | Q02750 |
| Molecular weight: | 45kDa |
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