Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody (AP0075) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-MEF2C-S396 Rabbit pAb
Catalog No:AB-AP0075
Manufacturer:ABclonal
Synonyms:MEF2C;C5DELq14.3;DEL5q14.3
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-MEF2C-S396 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. |
Immunogen: | A synthetic phosphorylated peptide around S396 of human MEF2C (NP_002388.2). |
Gene ID: | 4208 |
Swiss prot: | Q06413 |
Molecular weight: | 44kDa/47kDa/50kDa/51kDa/52kDa |
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