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Western blot analysis of extracts of HeLa cells, using Phospho-MCM2-S139 antibody (AP1192) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37℃.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-MCM2-S139 Rabbit pAb
Catalog No：AB-AP1192
Manufacturer：ABclonal
Synonyms：MCM2;BM28;CCNL1;CDCL1;D3S3194;DFNA70;MITOTIN;cdc19
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-MCM2-S139 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| Immunogen: | A synthetic phosphorylated peptide around S139 of human MCM2. |
| Gene ID: | 4171 |
| Swiss prot: | P49736 |
| Molecular weight: | 101kDa |
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