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Western blot analysis of extracts of 293T cells, using Phospho-MAP3K5-T845 antibody (AP1215) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-MAP3K5-T845 Rabbit pAb
Catalog No：AB-AP1215
Manufacturer：ABclonal
Synonyms：A; AS; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-MAP3K5-T845 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T845 of human MAP3K5. |
| Gene ID: | 26408 |
| Swiss prot: | O35099 |
| Molecular weight: |
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