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Western blot analysis of extracts of various cell lines, using Phospho-MAP3K5-S83 antibody (AP0059) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour, treated by TNF-ɑ (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-MAP3K5-S83 Rabbit pAb
Catalog No：AB-AP0059
Manufacturer：ABclonal
Synonyms：MAP3K5;ASK1;MAPKKK5;MEKK5
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-MAP3K5-S83 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S83 of human MAP3K5 |
| Gene ID: | 4217 |
| Swiss prot: | Q99683 |
| Molecular weight: | 69kDa/154kDa |
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