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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-LRRK2-S935 Rabbit mAb (AP1154) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Western blot analysis of extracts of C6 cells, using Phospho-LRRK2-S935 Rabbit mAb (AP1154) at 1:1000 dilution.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-LRRK2-S935 Rabbit mAb
Catalog No：AB-AP1154
Manufacturer：ABclonal
Synonyms：AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-LRRK2-S935 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S935 of human LRRK2 |
| Gene ID: | 120892 |
| Swiss prot: | Q5S007 |
| Molecular weight: | 286kDa |
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