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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-LDHA-Y10 antibody (AP0889) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-LDHA-Y10 Rabbit pAb
Catalog No：AB-AP0889
Manufacturer：ABclonal
Synonyms：GSD11;HEL-S-133P;LDHM;PIG19;LDHA
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-LDHA-Y10 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y10 of human LDHA. |
| Gene ID: | 3939 |
| Swiss prot: | P00338 |
| Molecular weight: | 37kDa |
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