Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (AP1142) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (AP1142) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-JunD-S255 Rabbit mAb
Catalog No:AB-AP1142
Manufacturer:ABclonal
Synonyms:AP-1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-JunD-S255 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S255 of human Phospho-JunD-S255 |
Gene ID: | 3727 |
Swiss prot: | P17535 |
Molecular weight: | 38,42kDa |
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