Western blot analysis of extracts of 293T cells, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 antibody (AP1163) at 1:1000 dilution.293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb
Catalog No:AB-AP1163
Manufacturer:ABclonal
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016] |
Immunogen: | A synthetic phosphorylated peptide around T183 & Y185 of human JNK1/2/3 (NP_620637.1). |
Gene ID: | 5599/5601/5602 |
Swiss prot: | P45983/P45984/P53779 |
Molecular weight: | 35kDa/44kDa/48kDa/27kDa/52kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-AP0026
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0020
Manufacturer:ABclonal
Synonyms:CRE-BP1;CREB-2;CREB2;HB16;TREB7;ATF2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0010
Manufacturer:ABclonal
Synonyms:BAD;BBC2;BCL2L8;Bad
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0006
Manufacturer:ABclonal
Synonyms:AAA;ABETA;ABPP;AD1;APPI;CTFgamma;CVAP;PN-II;PN2;Amyloid beta A4;APP;Aβ42;preA4
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0004
Manufacturer:ABclonal
Synonyms:AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0001
Manufacturer:ABclonal
Synonyms:ABL;JTK7;p150;c-ABL;v-abl;CHDSKM;c-ABL1;ABL1;c-Abl;bcr/abl
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00