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Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb
Catalog No：AB-AP0631
Manufacturer：ABclonal
Synonyms：JNK1/JNK2/JNK3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €525,00
| Product Name: | Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3 |
| Gene ID: | 5599/5601/5602 |
| Swiss prot: | P45983/P45984/P53779 |
| Molecular weight: | 35kDa/44kDa/48kDa/27kDa/52kDa |
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