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Western blot analysis of extracts of various cell lines, using Phospho-IkBɑ-S36 Rabbit mAb (AP0999) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded human colon using Phospho-IkBɑ-S36 Rabbit mAb (AP0999) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-IkBɑ-S36 Rabbit mAb (AP0999) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using Phospho-IkBɑ-S36 Rabbit mAb (AP0999) at dilution of 1:100 (40x lens).
Phospho-IκBα-S36 Rabbit mAb
Catalog No：AB-AP0999
Manufacturer：ABclonal
Synonyms：IKBA, MAD-3, NFKBI
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-I魏B伪-S36 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S36 of human I魏B伪 |
| Gene ID: | 4792 |
| Swiss prot: | P25963 |
| Molecular weight: | 39kDa |
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