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Western blot analysis of extracts of various cell lines, using Phospho-IRF7-S471/472 antibody (AP1264) at 1:500 dilution.293T and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-IRF7-S471/472 Rabbit pAb
Catalog No：AB-AP1264
Manufacturer：ABclonal
Synonyms：IRF7;IMD39;IRF-7H;IRF7A;IRF7B;IRF7C;IRF7H
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-IRF7-S471/472 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S471/472 of Human IRF7. |
| Gene ID: | 3665 |
| Swiss prot: | Q92985 |
| Molecular weight: | 18kDa/51kDa/54kDa/55kDa |
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