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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts of 293T cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-IRF3-S396 Rabbit pAb
Catalog No：AB-AP0623
Manufacturer：ABclonal
Synonyms：IIAE7;IRF3
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-IRF3-S396 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S396 of human IRF3 |
| Gene ID: | 3661 |
| Swiss prot: | Q14653 |
| Molecular weight: | 12kDa/16kDa/33kDa/47kDa/49kDa |
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