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Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-IRE1-S724 antibody (AP0878) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using Phospho-IRE1-S724 antibody (AP0878) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRE1-S724 antibody (AP0878) at 1:500 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-IRE1-S724 Rabbit pAb
Catalog No：AB-AP0878
Manufacturer：ABclonal
Synonyms：ERN1;IRE1;IRE1P;IRE1a;hIRE1p
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-IRE1-S724 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding s724 of human IRE1alpha. |
| Gene ID: | 2081 |
| Swiss prot: | O75460 |
| Molecular weight: | 6kDa/109kDa |
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