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Western blot analysis of extracts of HeLa cells, using Phospho-IRE1-S724 antibody (AP1146) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 300s.
Phospho-IRE1-S724 Rabbit mAb
Catalog No：AB-AP1146
Manufacturer：ABclonal
Synonyms：IRE1, IRE1P, IRE1a, hIRE1p
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-IRE1-S724 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S724 of human IRE1 |
| Gene ID: | 2081 |
| Swiss prot: | O75460 |
| Molecular weight: | 110kDa |
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