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Western blot analysis of extracts of 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 antibody (AP0044) at 1:1000 dilution. 293 cells were treated by EGF (25ug/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb
Catalog No：AB-AP0044
Manufacturer：ABclonal
Synonyms：IGF1R/INSR
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y1150 of human INSR and Y1135 of human IGF1R |
| Gene ID: | 3480/3643 |
| Swiss prot: | P08069/P06213 |
| Molecular weight: | 154kDa/155kDa/156kDa |
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