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Western blot analysis of extracts of various cell lines, using Phospho-IKKγ-S376 antibody (AP1111) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts of HeLa cells, using Phospho-IKKγ-S376 pAb (AP1111) at 1:1000 dilution or IKKγ antibody (A0917).HeLa cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-IKKγ-S376 Rabbit pAb
Catalog No：AB-AP1111
Manufacturer：ABclonal
Synonyms：IKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9; NF-kappa-B essential modulator
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-IKK纬-S376 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S376 of human IKK纬. |
| Gene ID: | 8517 |
| Swiss prot: | Q9Y6K9 |
| Molecular weight: | 36kDa/48kDa/55kDa |
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