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Western blot analysis of extracts of HeLa cells, using Phospho-IKKɑ-S180 antibody (AP0506) at 1:1000 dilution. HeLa cells were treated by both TNF-ɑ(20ng/mL) and Calyculin A (100nM) for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-IKKα-S180 Rabbit pAb
Catalog No：AB-AP0506
Manufacturer：ABclonal
Synonyms：CHUK;IKBKA;IKK-alpha;IKK1;IKKA;NFKBIKA;TCF16;IKK alpha
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-IKK伪-S180 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. |
| Immunogen: | A synthetic phosphorylated peptide around S180 of human IKK伪 (NP_001269.3). |
| Gene ID: | 1147 |
| Swiss prot: | O15111 |
| Molecular weight: | 84kDa |
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