Western blot analysis of extracts of HeLa cells, using Phospho-IKKɑ/β-S176/180 antibody (AP0891) at 1:1000 dilution.HeLa cells were treated by TNF-ɑ (20 ng/mL) and Calyculin A (100 nM) at 37℃ for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-IKKα/β-S176/180 Rabbit pAb
Catalog No:AB-AP0891
Manufacturer:ABclonal
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-IKK伪/尾-S176/180 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S176/180 of human IKK伪/尾. |
Gene ID: | 1147/3551 |
Swiss prot: | |
Molecular weight: |
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