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Western blot analysis of extracts of various cell lines, using Phospho-IkBɑ-S32 antibody (AP1220) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by TNF-ɑ (20 ng/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-IκBα-S32 Rabbit pAb
Catalog No：AB-AP1220
Manufacturer：ABclonal
Synonyms：IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-I魏B伪-S32 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S32 of human NFKBIA. |
| Gene ID: | 4792 |
| Swiss prot: | P25963 |
| Molecular weight: | 35kDa |
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