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Western blot analysis of extracts of HeLa cells, using Phospho-IkBɑ-S32 antibody (AP0731) at 1:1000 dilution. HeLa cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Western blot analysis of extracts of HeLa cells, using Phospho-IkBɑ-S32 pAb (AP0731) at 1:1000 dilution or IKB alpha antibody (A11397).HeLa cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded human colon using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Phospho-IkBɑ-S32 Rabbit pAb (AP0731) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Phospho-IκBα-S32 Rabbit pAb
Catalog No：AB-AP0731
Manufacturer：ABclonal
Synonyms：IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-I魏B伪-S32 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. |
| Immunogen: | A synthetic phosphorylated peptide around S32 of human I魏B伪 (NP_065390.1). |
| Gene ID: | 4792 |
| Swiss prot: | P25963 |
| Molecular weight: | 35kDa |
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