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Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Western blot analysis of extracts of PC-12 cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution.PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-HSP27/HSPB1-S82 Rabbit mAb
Catalog No：AB-AP1031
Manufacturer：ABclonal
Synonyms：CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-HSP27/HSPB1-S82 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S82 of human HSP27/HSPB1 |
| Gene ID: | 3315 |
| Swiss prot: | P04792 |
| Molecular weight: | 27kDa |
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