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Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using Phospho-HSP27/HSPB1-S78 antibody (AP0236).
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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-HSP27/HSPB1-S78 antibody (AP0236).
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Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S78 antibody (AP0236) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by serum-starvation overnight or treated by Anisomycin (25ug/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S78 pAb (AP0236) at 1:1000 dilution or HSP27/HSPB1 antibody (A16332).HeLa cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-HSP27/HSPB1-S78 Rabbit pAb
Catalog No：AB-AP0236
Manufacturer：ABclonal
Synonyms：HSPB1;CMT2F;HEL-S-102;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27
Applications：WB,IHC,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-HSP27/HSPB1-S78 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S78 of human HSP27/HSPB1 |
| Gene ID: | 3315 |
| Swiss prot: | P04792 |
| Molecular weight: | 22kDa |
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