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Western blot analysis of extracts of MCF7 cells, using Phospho-HSF1-S326 antibody (AP1189) at 1:500 dilution.MCF7 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-HSF1-S326 Rabbit pAb
Catalog No：AB-AP1189
Manufacturer：ABclonal
Synonyms：HSTF1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-HSF1-S326 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S326 of human HSF1 |
| Gene ID: | 3297 |
| Swiss prot: | Q00613 |
| Molecular weight: | 82kDa |
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