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Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (A1131).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-Histone H4-S1 Rabbit pAb
Catalog No：AB-AP0901
Manufacturer：ABclonal
Synonyms：H4FA;Histone H4;HIST1H4A;HIST4H4;H4/p
Applications：WB,IHC
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Histone H4-S1 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S1 of human Histone H4. |
| Gene ID: | 8359 |
| Swiss prot: | P62805 |
| Molecular weight: | 11kDa |
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