.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb (AP0846) at 1:1000 dilution.C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.C6 and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb (AP0846) at 1:1000 dilution or Histone H3 antibody (A2348).Both 293T cells and Raw264.7 cells were treated by Nocodazole (100 ng/mL) at 37℃ for 16 hours.C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Histone H3-T3 Rabbit pAb
Catalog No：AB-AP0846
Manufacturer：ABclonal
Synonyms：H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A
Applications：WB
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Histone H3-T3 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1). |
| Gene ID: | 8350 |
| Swiss prot: | P68431 |
| Molecular weight: | 15kDa |
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