Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S28 antibody (AP0097) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Histone H3-S28 Rabbit pAb
Catalog No:AB-AP0097
Manufacturer:ABclonal
Synonyms:H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A
Applications:WB
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Histone H3-S28 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
Immunogen: | A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003528.1). |
Gene ID: | 8350 |
Swiss prot: | P68431 |
Molecular weight: | 15kDa |
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