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Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody (AP0896) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour and treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0896) at 1:1000 dilution or Histone H3 antibody (A15741).HeLa,NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody (AP0896) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody (AP0896) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody (AP0896) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 Rabbit pAb (AP0896) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 Rabbit pAb (AP0896) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb (AP0896) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Phospho-Histone H3-S10/T11 Rabbit pAb
Catalog No：AB-AP0896
Manufacturer：ABclonal
Synonyms：H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Histone H3-S10/T11 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S10/T11 of human Histone H3. |
| Gene ID: | 8350 |
| Swiss prot: | P68431 |
| Molecular weight: | 15kDa |
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