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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HDAC5-S498 antibody (AP0202) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-HDAC5-S498 antibody (AP0202).
Phospho-HDAC5-S498 Rabbit pAb
Catalog No：AB-AP0202
Manufacturer：ABclonal
Synonyms：HDAC5;HD5;NY-CO-9
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-HDAC5-S498 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S498 of human HDAC5 |
| Gene ID: | 10014 |
| Swiss prot: | Q9UQL6 |
| Molecular weight: | 112kDa/121kDa/122kDa |
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