Western blot analysis of extracts of 293T cells, using Phospho-GSK3β-Y216 antibody (AP0512) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb
Catalog No:AB-AP0512
Manufacturer:ABclonal
Synonyms:GSK3B;gsk-3β
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-GSK3尾-Y216 + GSK3伪-Y279 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic phosphorylated peptide around Y216 of human GSK3尾 (NP_001139628). |
Gene ID: | 2932 |
Swiss prot: | P49841 |
Molecular weight: | 46kDa/48kDa |
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