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Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3ɑ-S21 pAb (AP0582). Western blot was performed from the immunoprecipitate using Phospho-GSK3ɑ-S21 pAb (AP0582) at a dilition of 1:1000. 293T cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.
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Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3ɑ-S21 antibody (AP0582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
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Western blot analysis of extracts of 293 cells, using Phospho-GSK3ɑ-S21 antibody (AP0582) at 1:1000 dilution or GSK3ɑ antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-GSK3α-S21 Rabbit pAb
Catalog No：AB-AP0582
Manufacturer：ABclonal
Synonyms：GSK3 alpha;GSK3A
Applications：WB,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-GSK3伪-S21 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer’s disease. |
| Immunogen: | A synthetic phosphorylated peptide around S21 of human GSK3伪 (NP_063937.2). |
| Gene ID: | 2931 |
| Swiss prot: | P49840 |
| Molecular weight: | 50kDa |
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