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Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 antibody (AP0039) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 pAb (AP0039) at 1:2000 dilution or GSK3β antibody (A2081).HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Phospho-GSK3β-S9 Rabbit pAb
Catalog No：AB-AP0039
Manufacturer：ABclonal
Synonyms：GSK3B;gsk-3β
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-GSK3尾-S9 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S9 of human GSK3尾 (NP_001139628.1). |
| Gene ID: | 2932 |
| Swiss prot: | P49841 |
| Molecular weight: | 46kDa/48kDa |
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