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Western blot analysis of extracts of various cell lines, using Phospho-GSK3β-S9 antibody (AP1258) at 1:1000 dilution.HeLa NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-GSK3β-S9 Mouse mAb
Catalog No：AB-AP1258
Manufacturer：ABclonal
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Mouse
Conjugate：No
€185,00 – €525,00
| Product Name: | Phospho-GSK3尾-S9 Mouse mAb |
| Category: | Phosphorylated Antibodies |
| Description: | |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S9 of human GSK3尾 |
| Gene ID: | |
| Swiss prot: | |
| Molecular weight: |
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