Western blot analysis of extracts of SH-SY5Y cells, using Phospho-GluR1/GRIA1-S845 antibody (AP0825) at 1:1000 dilution or GluR1/GRIA1 antibody (A1826). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Phospho-GluR1/GRIA1-S845 Rabbit pAb
Catalog No:AB-AP0825
Manufacturer:ABclonal
Synonyms:GRIA1;GLUH1;GLUR1;GLURA;GluA1;HBGR1;GluR1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-GluR1/GRIA1-S845 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S845 of human GluR1/GRIA1 |
Gene ID: | 2890 |
Swiss prot: | P42261 |
Molecular weight: | 92kDa/93kDa/101kDa/102kDa |
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