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Western blot analysis of extracts of HeLa cells, using Phospho-Glucocorticoid Receptor-S211 antibody (AP0759) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-Glucocorticoid Receptor-S211 Rabbit pAb
Catalog No：AB-AP0759
Manufacturer：ABclonal
Synonyms：GCCR;GCR;GCRST;GR;GRL;NR3C1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Glucocorticoid Receptor-S211 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). |
| Immunogen: | A synthetic phosphorylated peptide around S211 of human Glucocorticoid Receptor (NP_000167.1). |
| Gene ID: | 2908 |
| Swiss prot: | P04150 |
| Molecular weight: | 49-85kDa |
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