Western blot analysis of extracts of K-562 cells, using Phospho-FOXO3A-S425 antibody (AP1200) at 1:1000 dilution.K-562 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-FOXO3A-S425 Rabbit pAb
Catalog No:AB-AP1200
Manufacturer:ABclonal
Synonyms:AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€150,00 – €410,00
Product Name: | Phospho-FOXO3A-S425 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthetic phosphorylated peptide around S425 of human FOXO3A. |
Gene ID: | 2309 |
Swiss prot: | O43524 |
Molecular weight: |
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