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Western blot analysis of extracts of C6 cells, using Phospho-Filamin A-S2152 antibody (AP0783) at 1:1000 dilution.C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
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Western blot analysis of extracts of HeLa cells, using Phospho-Filamin A-S2152 pAb (AP0783) at 1:1000 dilution or Filamin A antibody (A7896).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
Phospho-Filamin A-S2152 Rabbit pAb
Catalog No：AB-AP0783
Manufacturer：ABclonal
Synonyms：FLNA;ABP-280;ABPX;CSBS;CVD1;FLN;FLN-A;FLN1;FMD;MNS;NHBP;OPD;OPD1;OPD2;XLVD;XMVD;filamin-A
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Filamin A-S2152 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S2152 of human Filamin A (NP_001104026.1). |
| Gene ID: | 2316 |
| Swiss prot: | P21333 |
| Molecular weight: | 280kDa |
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