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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-ESRɑ-S118 antibody (AP0146).
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Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-ESRɑ-S118 antibody (AP0146).
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Western blot analysis of extracts of MCF7 cells, using Phospho-ESRɑ-S118 pAb (AP0146) at 1:1000 dilution.MCF7 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-ESRα-S118 Rabbit pAb
Catalog No：AB-AP0146
Manufacturer：ABclonal
Synonyms：ESR1;ER;ESR;ESRA;ESTRR;Era;NR3A1;ER alpha
Applications：WB,IHC,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ESR伪-S118 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S118 of human Estrogen Receptor alpha (ESR1) |
| Gene ID: | 2099 |
| Swiss prot: | P03372 |
| Molecular weight: | 35kDa/47kDa/53kDa/66kDa |
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