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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ESRɑ-S118 Rabbit mAb (AP1004) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-ESRα-S118 Rabbit mAb
Catalog No：AB-AP1004
Manufacturer：ABclonal
Synonyms：ER, ESR, ESRA, ESTRR, Era, NR3A1
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-ESR伪-S118 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S118 of human Estrogen Receptor alpha (ESR1) |
| Gene ID: | 2099 |
| Swiss prot: | P03372 |
| Molecular weight: | 66kDa |
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