Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (AP0051) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-ERK3-S189 Rabbit pAb
Catalog No:AB-AP0051
Manufacturer:ABclonal
Synonyms:MAPK6;ERK3;HsT17250;PRKM6;p97MAPK
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-ERK3-S189 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. |
Immunogen: | A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1). |
Gene ID: | 5597 |
Swiss prot: | Q16659 |
Molecular weight: | 82kDa |
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