Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-Y204 antibody (AP0235) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-ERK1-Y204 Rabbit pAb
Catalog No:AB-AP0235
Manufacturer:ABclonal
Synonyms:ERK-1;ERK1;ERT2;HS44KDAP;HUMKER1A;P44ERK1;P44MAPK;PRKM3;p44-ERK1;p44-MAPK;MAPK3
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-ERK1-Y204 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. |
Immunogen: | A synthetic phosphorylated peptide around Y204 of human ERK1 (NP_002737.2). |
Gene ID: | 5595 |
Swiss prot: | P27361 |
Molecular weight: | 38kDa/40kDa/43kDa |
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